evaluation of resistance six potato genotypes to early blight disease caused by the pathogenic fungus alternaria tenuissima and alternaria allternata under in vitro and greenhouse conditions

نویسندگان

حمیدرضا میرکریمی

دانشجوی دکتری اصلاح نباتات دانشگاه آزاد اسلامی تهران واحد علوم و تحقیقات احمد عباسی مقدم

استادیار گروه بیماری شناسی گیاهی، بانک ژن گیاهی ملی ایران جواد مظفری

دانشیار گروه بیماری شناسی گیاهی، بانک ژن گیاهی ملی ایران

چکیده

in order to determine the resistance levels and the most suitable potato genotypes to the early blight, an experiment was conducted in a completely randomized factorial design with three replications, in national plant gene bank of iran during 2007-2008. factors included pathogenic fungus, a. tenuissima and a. alternata and six potato genotypes, picasso, marfona, delta, agria, cosmos and armida. analysis of variance, in vitro and greenhouse evaluation showed that there were significant between pathogen, genotype and their interactions. in vitro evaluation, symptoms appeared after the second day and continued until the sixth day. based on area under the disease progress curve for pathogenic a. alternata, cosmos genotype had a lower level of agria (susceptible control), while picasso and marfona with delta (resistant control) were resistant patients.with the pathogenic a. tenuissima, cosmos was sensitive, and only picasso was resistant patient.the method greenhouse, symptoms began on the third day and it lasted until the twentieth day. in this evaluation, using both fungal isolates, cosmos was sensitive level and picasso was resistant level. in vitro and greenhouse assessment, armida not show any signs of disease, it may be referred to as a genotype-immune disease, after field testing. since, the results of the assessment of resistance was generally similar in both in vitro and greenhouse conditions, it can be argued that this study is validated by classifying genotypes to resistant and susceptible.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

ارزیابی نمونه‌های ژنتیکی دیپلوید وحشی کلکسیون سیب‌زمینی بانک ژن گیاهی ملی ایران برای مقاومت به بیماری لکه موجی ناشی از Alternaria tenuissima وAlternaria solani در شرایط درون شیشه‌ای و گلخانه‌ای

Species of pathogenic fungi Alternaria SPP developed early blight disease on potato. In order to evaluate the resistance levels of wild diploid accessions of National Plant Gene bank of Iran, a factorial randomized complete design with three replications was performed in National Plant Gene Bank under in vitro and greenhouse condition during 2007-2008 and disease symptoms were recorded. Based o...

متن کامل

Evaluation of Citrus Cultivars Susceptibility to Leaf Spot Disease Caused by Alternaria alternata under in vitro Conditions

Alternaria brown spot is a serious disease of fruit and foliage of citrus. This disease caused by Alternaria alternata and causes serious economical losses on susceptible cultivars in Iran. In this research, susceptibility of seven citrus cultivars including Fortune, Orlando tangelo, Mineola tangelo, Page, Clementine, Sour orange and Sweet orange (Thomson) fruits to A. alternata was investigate...

متن کامل

Morphological, pathogenic, and molecular characterization of alternaria isolates associated with alternaria late blight of pistachio.

ABSTRACT Alternaria isolates were obtained from various pistachio tissues collected in five orchards in California. For all isolates, morphological characteristics of the colony and sporulation apparatus were determined and compared with those of representative isolates of A. alternata, A. tenuissima, A. arborescens, and A. infectoria. A selection of the pistachio isolates and the representativ...

متن کامل

Tenuazonic acid production by Alternaria alternata and Alternaria tenuissima isolated from cotton.

Cultures of Alternaria alternata (three isolates) and Alternaria tenuissima (three isolates) obtained from cottonseeds and bolls were toxigenic when cultured on various laboratory media. A mycotoxin was isolated and identified as tenuazonic acid by using solvent partition, thin-layer chromatography, and instrument analyses. Toxicity was monitored with brine shrimp and chicken embryo bioassays. ...

متن کامل

Pilot-plant production of protease by Alternaria tenuissima.

Agar slants of a selected Alternaria tenuissima strain were illuminated to give conidia suitable for further propagation. For production of protease with an optimal caseolytic activity in the region of pH 8 to 10, the fungus was cultivated in steel fermentors with 6- and 60-liter working capacity. Maximal activity, 1.5 enzyme units per liter, was attained in a medium based on liver after about ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
پژوهش های تولید گیاهی

جلد ۲۰، شماره ۴، صفحات ۱۱۷-۱۳۲

کلمات کلیدی
in order to determine the resistance levels and the most suitable potato genotypes to the early blight an experiment was conducted in a completely randomized factorial design with three replications in national plant gene bank of iran during 2007 2008. factors included pathogenic fungus a. tenuissima and a. alternata and six potato genotypes picasso marfona delta agria cosmos and armida. analysis of variance in vitro and greenhouse evaluation showed that there were significant between pathogen genotype and their interactions. in vitro evaluation symptoms appeared after the second day and continued until the sixth day. based on area under the disease progress curve for pathogenic a. alternata cosmos genotype had a lower level of agria (susceptible control) while picasso and marfona with delta (resistant control) were resistant patients.with the pathogenic a. tenuissima cosmos was sensitive and only picasso was resistant patient.the method greenhouse symptoms began on the third day and it lasted until the twentieth day. in this evaluation using both fungal isolates cosmos was sensitive level and picasso was resistant level. in vitro and greenhouse assessment armida not show any signs of disease it may be referred to as a genotype immune disease after field testing. since the results of the assessment of resistance was generally similar in both in vitro and greenhouse conditions it can be argued that this study is validated by classifying genotypes to resistant and susceptible.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023